The new fuel storage agreement includes two key depots in Scotland – at Campbeltown and on Loch Striven.
Both the depots are already supporting the UK Royal Fleet Auxiliary (RFA) and the RN operations.
The initial duration of the agreement is five years and includes an automatic five-year extension period.
The extension period will allow revisions, further strengthening international cooperation and allied operations in the North Atlantic and High North regions.
The agreement will allow the RN to purchase, deliver, store as well as issue bunker and cargo fuel at both the depots on behalf of the US Navy and the Military Sealift Command (MSC).
The RFA’s counterpart, MSC is a crucial support and supply arm of the US Navy.
Apart from supplying to the US and MSC vessels, Campbeltown and Loch Striven depots will continue to hold and issue fuel to the RN and RFA vessels.
The depots will also continue provisioning the navies of other allied nations.
The Anglo-American agreement showcases close logistics cooperation between the RN and the US Navy, supporting interoperability and integration agreement signed by London and Washington.
Oil and Pipelines Agency, which manages Naval Oil Fuel Depots, chief executive Adrian Jackson said: “The Campbeltown and Loch Striven Oil Fuel Depots are thrilled to have American fuel once again and both depots will be storing F-76 and F-44 for the US.
“Several visits were held with US Navy representatives last year and they herald a future strong and reciprocal professional relationship. We look forward to a long and fruitful relationship.”